S.F. A number of strains of heterotrophic bacteria were isolated from various environments on the basis of their potential to oxidize inorganic sulfur compounds to tetrathionate. Under anaerobic conditions, the rate of sulfide oxidation was very low. A. Sequence analysis of the 16S rRNA gene of four selected isolates showed their affiliation to specific genomovars of Pseudomonas stutzeri and the proposed new species, Pseudomonas balearica. Search for more papers by this author. Maniatis B.L. Many of them could grow anaerobically with acetate and nitrate, and eight strains could oxidize thiosulfate to tetrathionate under the same conditions. J. Both of these compounds are known to inhibit N2O reductase in many organisms [35], and therefore N2O should accumulate at the interface. The enrichment culture developed in two phases. The S-type predominated on wet surfaces and during anaerobic incubation. This work received financial support from the Max-Planck-Gesellschaft, Munich to A.T. Tuttle lutescens’ grown anaerobically with acetate, thiosulfate and either nitrate or N2O could oxidize thiosulfate anaerobically, but were totally inactive with nitrite as electron acceptor. The carbon in the CH 3 group in the acetic acid formed in this reaction has the same oxidation state as it did in the starting material: -3. P.A. R. 0000026310 00000 n H. FEMS Microbiol Ecol 19:117–125, Sorokin DY, Tourova TP, Sjollema KA, Kuenen JG (2003) Thialkalivibrio nitratireducens sp. 16S rRNA sequence analysis showed that isolates ChG 5-2, ChG 5-3, BG 2 and TG 3 were new Pseudomonas stutzeri strains. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Cytochrome b was present at relatively low concentrations, compared to the level of cytochrome c. CO difference spectra of dithionite-reduced extracts had the maxima and minima in the γ region (415 and 432 nm respectively), and minima in the α region (556–558 nm) typical of cytochrome oxidase type o. <> The observed preference for N2O as an electron acceptor for anaerobic thiosulfate oxidation to tetrathionate during organotrophic growth could be explained by the high redox potential of N2O/N2, compared with the nitrate/nitrite and nitrite/N2O couples (ΔE0′=1.36, 0.43 and 0.36 V, respectively), and assuming a relatively high value for the S2O32−/S4O62− pair (about 0.1 V). The synthesis of ATP during the oxidation of thiosulfate to tetrathionate in washed suspensions of cells from several strains has been reported [34]. For example, during the anaerobic growth of strain TG 3 with nitrate and acetate, there was no evidence of biomass increase when thiosulfate was added, even under acetate limitation in continuous culture (Table 3). (, Ursing Of the three chalcopyrite concentrates used in this work; viz., Opemiska, Bethlehem and Lornex, best results were obtained with the Opemiska concentrate. 1, 28359 Bremen, Germany, Kluyver Laboratory of Biotechnology, TU Delft, Julianalaan 67, 2628 BC Delft, The Netherlands, Metabolism of thiosulfate and tetrathionate by heterotrophic bacteria, Dissimilatory reduction of inorganic sulfur by facultatively anaerobic marine bacteria, Bacterial oxidation of thiosulfate at the Black Sea, Oxidation of inorganic sulfur species by heterotetrophic microorganisms, Isolation and characterization of alkaliphilic heterotrophic bacteria oxidizing sulfur compounds to tetrathionate, Reduction of tetrathionate and thiosulfate and oxidation of sulfide in, Oxidation of thiosulfate to tetrathionate by marine pseumonad strain ChG 7-3: influence on growth and properties of oxidizing system, Sulfide and thiosulfate oxidizing bacteria in anoxic marine basins, Chemolithotrophic sulfur oxidizing bacteria from Galapagos rift hydrothermal vents, The spectrophotometric determination of nitrate and nitrate in natural waters with particular reference to seawater, Rapid micromethod for determination of nitrate in presence of nitrite for biochemical studies, Cyanolysis and spectrophotomentric estimation of trithionate in mixture with thiosulfate and tetrathionate, A procedure for isolation of DNA from microorganisms, The quantitative measurements of DNA-DNA hybridization from renaturation rates, Labelling of DNA to high specific activity in vivo by nick translation with DNA polymerase I, Cold Spring Harbor Laboratory Press, Cold Spring Harbor. Cells were centrifuged, washed and resuspended in 0.05 M potassium phosphate buffer (pH 7.5) with 15 g l−1 NaCl and disrupted by sonication. /ID[<2A5105CFED2BF522AD95A9F46E43B19B><83672BE44D18010DAC36741C3C9B715F>] Others functioned best with N2O during anaerobic thiosulfate oxidation. If cultures were grown aerobically, they could not denitrify with thiosulfate as the electron donor, even though low levels of the nitrate and nitrite reductases could be detected if acetate was supplied. J. Google Scholar, Vedenina IY, Sorokin DY (1992) ATP synthesis during oxidation of thiosulfate to tetrathionate by heterotrophic bacteria. Copyright © 1999 Federation of European Microbiological Societies. (, Muyzer Kuenen Difference spectra of cell-free extracts prepared from strains from group 1 showed the presence of cytochrome cd1. 0000026134 00000 n Ghosh W(1), Roy P. Author information: (1)Department of Microbiology, Bose Institute, Kolkata, India. Chambers Kelly B.M. C. The isolates described here differ from the chemolithotrophic sulfur bacteria in that they produce tetrathionate rather than sulfate. /Prev 266297 (, Rainey Where there was a low similarity level (less than 50%), the nick translation technique with 3H-labelled DNA was also used [22]. J. In the presence of nitrate or N2O, thiosulfate was oxidized exclusively to tetrathionate (Table 2). W. Cytochrome cd1 nitrite reductase was not spectroscopically detected in cell-free extracts prepared from cells grown anaerobically with acetate and nitrate. C.O. Rhodes Fuji McCaughey Group 1 includes strains ChG 4-1, ChG 5-2, ChG 5-3 and ChG 7-4, all of which had highly active cytochrome cd1 nitrite reductase (see below). Tetrathionate is also produced as an intermediate in the oxidation of thiosulfate by several Beta- and Gammaproteobacteria (Kelly et al., 1997; Ghosh et al., 2005), but not by any Alphaproteobacteria, though quite a few members of the latter use tetrathionate as a chemolithotrophic substrate (Kelly et al., 2000; Deb et al., 2004). ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Anaerobic oxidation of thiosulfate to tetrathionate by obligately heterotrophic bacteria, belonging to the, Heterotrophic tetrathionate-producing bacterium. Among the organic compounds utilized as carbon and energy sources were: organic acids including acetate, succinate, malate, α-ketoglutarate, citrate, fumarate, lactate, pyruvate, propionate, butyrate, gluconate, glyoxylate; alcohols including ethanol, propanol, glycerol; hexoses –d-glucose, d-fructose and d-maltose; amino acids and amines including i-alanine, l-glutamate, l-glutamine, l-aspartate, l-asparagine, l-proline and l-leucine. The presence of tetrathionate synthase, the enzyme responsible for thiosulfate oxidation, was detected in strain HG 1. Thiosulfate and tetrathionate were measured by cyanolysis [18]. Tetrathionate is an unstable compound (69) and thus would not be expected to persist in soils. Thiosulfate is produced by the chemical oxidation of sulfide at the low oxygen concentrations to be found in seawater at these interfaces [36]. Ursing DNA from E. coli gave less than 0.1% homology with any of these samples. E. Dimitry Y. Sorokin. In some experiments it even stimulated the reaction. N. Experiments with washed cells were done in 40-ml flasks containing 5 ml of cell suspension and sealed with butyl rubber stoppers. /T 266306 Robertson H.W. Extremophiles 9, 501–504 (2005). Katayama Two colony types were formed. 0000019624 00000 n Woese D.Yu. The scale bar corresponds to 5 substitutions per 1000 nucleotides. Given the increased thiosulfate measured in our experiments, we also expected to detect elevated tetrathionate in inflamed animals. When nitrate was supplied in excess, more thiosulfate was oxidized, but nitrite accumulation prevented complete conversion. The medium bottles and the gas phase of the fermenter were kept under a gentle argon flow. Correspondence to D.Yu. Inoue Motile cells were only found at the very edge of such colonies. Copyright © 2020 Elsevier B.V. or its licensors or contributors. Burkhardt N2O was the best electron acceptor for anaerobic thiosulfate oxidation by this group, and acetate did not inhibit anaerobic thiosulfate oxidation by the washed cells. 0000021153 00000 n As can be seen from Fig. The morphology of the cells within the R- and S-type colonies was sufficiently different to suggest two different species. /Info 56 0 R It is to be expected, as techniques are refined, that other strains will be classified, upon closer examination, as P. stutzeri.

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